Proteins produced with UNLOCK PICHIA® in peer-reviewed studies and research by our scientists.
Customers and partners have published peer-reviewed studies on proteins produced by VALIDOGEN or with our strains. These are the products behind the studies.
Chelator Conjugation Chemistry Matters: Squaramide Linker Enhances Tumor Contrast in 68Ga-labeled Anti-CEA Nanobody Tracer
Saliën J et al. · ACS Omega · 2026
Read the article: https://doi.org/10.1021/acsomega.5c11686
“This batch was produced in Pichia pastoris by VALIDOGEN GmbH (Grambach, Austria)” (methods section)
Improving the in vivo activity of glycosaminoglycan-binding immunomodulatory drugs by fusion to human serum albumin
Beretta F et al. · International Journal of Biological Macromolecules · 2026
Read the article: DOI: https://doi.org/10.1016/j.ijbiomac.2025.149825
The HSA-CXCL9(74–103) fusion protein was produced in Pichia pastoris cells by Validogen (Grambach, Austria).
Designing a CXCL8-hsa chimera as potential immunmodulator of the tumor micro-environment
Gerlza T et al. · Frontiers Immunology · 2025
Read the article: https://doi.org/10.3389/fimmu.2025.1539733
Pichia pastoris CBS7435 muts-PDI strain and fermentation by Validogen (Grambach, Austria).
A bispecific T cell engager recruits both type 1 NKT and Vγ9Vδ2T cells for the treatment of CD1d-expressing hematological malignancies
Lameris R et al. · Cell Reports Medicine · 2023
Read the article: https://doi.org/10.1016/j.xcrm.2023.100961
“expressed in P. pastoris (Validogen Gmbh, Graz, Austria)” (methods section)
Designing and developing a sensitive and specific SARS CoV2 RBD IgG detection kit
Raoufi E et al. · Clinica Chimica Acta · 2023
Read the article: https://doi.org/10.1016/j.cca.2023.117279.
The antigen was produced with VALIDOGEN's expression plasmid pPZ-aMFPAOX1WT and platform strain (methods section).
Preclinical development of a bispecific TNFα/IL23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease
Roberts KJ et al. · Scientific Reports · 2021
Read the article: https://doi.org/10.1038/s41598-021-97236-0
“from a Pichia pastoris mutS PDI strain (Validogen, Graz)” (methods section)
Equine keratinocytes in the pathogenesis of insect bite hypersensitivity: Just another brick in the wall?
Cvitas I et al. · PLOS ONE · 2022
“had been expressed in yeast Pichia pastoris, Validogen GMBH,(formerly VTU Technology), Grambach, ATˮ
Read the article: https://doi.org/10.1371/journal.pone.0266263
Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens
Novotny EN et al. · Allergy · 2021
“yeast Pichia pastoris, VALIDOGEN GMBH (formerly VTU Technology), Grambach, ATˮ
Read the article: https://doi.org/10.1111/all.14556
High Affinity Binders to EphA2 Isolated from Abdurin Scaffold Libraries; Characterization, Binding and Tumor Targeting
Ullman C et al. · Plos One · 2015
Read the article: https://doi.org/10.1371/journal.pone.0135278
Chelator Conjugation Chemistry Matters: Squaramide Linker Enhances Tumor Contrast in 68Ga-labeled Anti-CEA Nanobody Tracer
Saliën J et al. · ACS Omega · 2026
Read the article: https://doi.org/10.1021/acsomega.5c11686
“This batch was produced in Pichia pastoris by VALIDOGEN GmbH (Grambach, Austria)” (methods section)
Improving the in vivo activity of glycosaminoglycan-binding immunomodulatory drugs by fusion to human serum albumin
Beretta F et al. · International Journal of Biological Macromolecules · 2026
Read the article: DOI: https://doi.org/10.1016/j.ijbiomac.2025.149825
The HSA-CXCL9(74–103) fusion protein was produced in Pichia pastoris cells by Validogen (Grambach, Austria).
Designing a CXCL8-hsa chimera as potential immunmodulator of the tumor micro-environment
Gerlza T et al. · Frontiers Immunology · 2025
Read the article: https://doi.org/10.3389/fimmu.2025.1539733
Pichia pastoris CBS7435 muts-PDI strain and fermentation by Validogen (Grambach, Austria).
A bispecific T cell engager recruits both type 1 NKT and Vγ9Vδ2T cells for the treatment of CD1d-expressing hematological malignancies
Lameris R et al. · Cell Reports Medicine · 2023
Read the article: https://doi.org/10.1016/j.xcrm.2023.100961
“expressed in P. pastoris (Validogen Gmbh, Graz, Austria)” (methods section)
Designing and developing a sensitive and specific SARS CoV2 RBD IgG detection kit
Raoufi E et al. · Clinica Chimica Acta · 2023
Read the article: https://doi.org/10.1016/j.cca.2023.117279.
The antigen was produced with VALIDOGEN's expression plasmid pPZ-aMFPAOX1WT and platform strain (methods section).
Preclinical development of a bispecific TNFα/IL23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease
Roberts KJ et al. · Scientific Reports · 2021
Read the article: https://doi.org/10.1038/s41598-021-97236-0
“from a Pichia pastoris mutS PDI strain (Validogen, Graz)” (methods section)
Equine keratinocytes in the pathogenesis of insect bite hypersensitivity: Just another brick in the wall?
Cvitas I et al. · PLOS ONE · 2022
“had been expressed in yeast Pichia pastoris, Validogen GMBH,(formerly VTU Technology), Grambach, ATˮ
Read the article: https://doi.org/10.1371/journal.pone.0266263
Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens
Novotny EN et al. · Allergy · 2021
“yeast Pichia pastoris, VALIDOGEN GMBH (formerly VTU Technology), Grambach, ATˮ
Read the article: https://doi.org/10.1111/all.14556
High Affinity Binders to EphA2 Isolated from Abdurin Scaffold Libraries; Characterization, Binding and Tumor Targeting
Ullman C et al. · Plos One · 2015
Read the article: https://doi.org/10.1371/journal.pone.0135278
Independent reviews and studies that reference VALIDOGEN, UNLOCK PICHIA® or our published work.
Petersson, M., Pettersen, J. S., Henriksen, H. B., Duzs, Á., Fernández-Quintero, M. L., Burlet, N. J., … Thrane, S. W. (2026). Attenuating ETEC virulence using a heat-labile enterotoxin–blocking binding protein. Gut Microbes, 18(1). https://doi.org/10.1080/19490976.2025.2597567
References VALIDOGEN case study III on methanol-induced bivalent VHH production.
Moraes, L.M.P.d.; Marques, H.F.; Reis, V.C.B.; Coelho, C.M.; Leitão, M.d.C.; Galdino, A.S.; Porto de Souza, T.P.; Piva, L.C.; Perez, A.L.A.; Trichez, D.; et al. Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology.J. Fungi 2024, 10, 411. https://doi.org/10.3390/jof10060411
Cites market data from a VALIDOGEN report and phytase results from our AOX1 promoter library.
Barone, G.D.; Emmerstorfer-Augustin, A.; Biundo, A.; Pisano, I.; Coccetti, P.; Mapelli, V.; Camattari, A. Industrial Production of Proteins with Pichia pastoris - Komagataella phaffii. Biomolecules 2023, 13, 441. https://doi.org/10.3390/biom13030441
Cites our 2017 white paper on methanol-free phytase production.
Bernauer L, Radkohl A, Lehmayer LGK and Emmerstorfer-Augustin A (2021): Komagataella phaffii as Emerging Model Organism in Fundamental Research. Front. Microbiol. 11:607028. https://doi.org/10.3389/fmicb.2020.607028
Describes the promoter libraries developed by VALIDOGEN (formerly VTU Technology) - marketed as UNLOCK PICHIA®.
Yagmur Unver, Ibrahim Dagci. Komagataella phaffii (Pichia pastoris) as a Powerful Yeast Expression System for Biologics Production. Front. Biosci. (Elite Ed) 2024, 16(2), 19. https://doi.org/10.31083/j.fbe1602019
Cites our 2017 white paper on methanol-free phytase production.
Methods in Molecular Biology (Clifton, N.J.)
2019-01-01 | Journal article
DOI: 10.1007/978-1-4939-9024-5_7
PMID: 30737740
Contributors: Weis R
International Journal of Biological Macromolecules
2025-12-26 | Journal article
DOI: 10.1016/j.ijbiomac.2025.149825
PMID: 41456776
Contributors: Beretta F; Agten L; de Oliveira VLS; Berghmans N; Trojacher C; Atlic A; Almer J; Struyf S; Weis R; Proost P et al.
Frontiers in Immunology
2025-03-07 | Journal article
DOI: 10.3389/fimmu.2025.1539733
PMID: 40124384
PMC: PMC11926544
Contributors: Gerlza T; Trojacher C; Fuchs T; Atlic A; Weis R; Adage T; Kungl AJ
Journal of Biotechnology
2017-08 | Journal article
DOI: 10.1016/j.jbiotec.2017.06.037
Part of ISSN: 0168-1656
Contributors: Martin Koller; Predrag Horvat; Gerhart Braunegg; Aid Atlic; Denis Vadlja
Neoplasia (New York, N.Y.)
2016-01-01 | Journal article
DOI: 10.1016/j.neo.2015.11.013
PMID: 26806351
PMC: PMC4735630
Contributors: Roblek M; Strutzmann E; Zankl C; Adage T; Heikenwalder M; Atlic A; Weis R; Kungl A; Borsig L
PloS one
2015-08-27 | Journal article
DOI: 10.1371/journal.pone.0135278
PMID: 26313909
PMC: PMC4552014
Contributors: Ullman C; Mathonet P; Oleksy A; Diamandakis A; Tomei L; Demartis A; Nardi C; Sambucini S; Missineo A; Alt K; Weis R et al.
Protein engineering, design & selection : PEDS
2015-05-11 | Journal article
DOI: 10.1093/protein/gzv025
PMID: 25969511
Contributors: Gerlza T; Winkler S; Atlic A; Zankl C; Konya V; Kitic N; Strutzmann E; Knebl K; Adage T; Heinemann A; Weis R et al.
Journal of biotechnology
2014-09-03 | Journal article
DOI: 10.1016/j.jbiotec.2014.08.035
PMID: 25193713
Contributors: Mellitzer A; Ruth C; Gustafsson C; Welch M; Birner-Grünberger R; Weis R; Purkarthofer T; Glieder A
Microbial cell factories
2012-05-14 | Journal article
DOI: 10.1186/1475-2859-11-61
PMID: 22583625
PMC: PMC3503753
Contributors: Mellitzer A; Weis R; Glieder A; Flicker K
Biotechnology Journal
2011-04-29 | Journal article
DOI: 10.1002/biot.201100001
PMID: 21538898
Contributors: Mellitzer A; Glieder A; Weis R; Reisinger C; Flicker K
Systems and Synthetic Biology
2010-09 | Journal article
DOI: 10.1007/s11693-010-9057-0
Part of ISSN: 1872-5325
Part of ISSN: 1872-5333
Contributors: C. Ruth; T. Zuellig; A. Mellitzer; R. Weis; V. Looser; K. Kovar; A. Glieder
Advanced Synthesis & Catalysis
2009-09 | Journal article
DOI: 10.1002/adsc.200900190
Part of ISSN: 1615-4150
Part of ISSN: 1615-4169
Contributors: Roland Weis; Margit Winkler; Matthias Schittmayer; Spiros Kambourakis; Mandy Vink; J. David Rozzell; Anton Glieder
Journal of biotechnology
2006-12-09 | Journal article
DOI: 10.1016/j.jbiotec.2006.12.003
PMID: 17234294
Contributors: Gaisberger R; Weis R; Luiten R; Skranc W; Wubbolts M; Griengl H; Glieder A
Journal of biotechnology
2006-12-09 | Journal article
DOI: 10.1016/j.jbiotec.2006.12.001
PMID: 17224199
Contributors: Weis R; Gaisberger R; Gruber K; Glieder A
Angewandte Chemie (International ed. in English)
2005-07-01 | Journal article
DOI: 10.1002/anie.200500435
PMID: 15999392
Contributors: Weis R; Gaisberger R; Skranc W; Gruber K; Glieder A
FEMS yeast research
2004-11-01 | Journal article
DOI: 10.1016/j.femsyr.2004.06.016
PMID: 15489201
Contributors: Weis R; Luiten R; Skranc W; Schwab H; Wubbolts M; Glieder A
