Speedy determination of protein expression potential in Pichia pastoris
Let us elucidate the expression potential of your protein in a quick feasibility study. Starting from an optimized DNA fragment we will clone, transform, and screen for expression in Pichia for a reliable go/no-go decision within a few weeks. In a targeted and concise approach we evaluate different molecular tools to find the optimum combination of genetic elements for your protein. Building on these factors, a highly efficient production strain can then be developed in a comprehensive strain development program by fully applying VALIDOGEN’s UNLOCK PICHIA® toolbox.
Positive strains can be directly transferred to a bioreactor cultivation enabling the production of initial protein samples. To provide you with a highly pure sample of your protein in a short time we will set up a purification process based on standard chromatography techniques, yielding milligram to gram quantities of pure protein from a Pichia culture supernatant.
Benefits
Our predictive high-throughput screening system quickly and effectively evaluates the expression potential of your protein. Customizable screening parameters allow for the assessment of UNLOCK PICHIA tools as desired.
Capable of monitoring and controlling temperature, pH value, level of dissolved oxygen, and feed rate, our 1 mL well microscale cultivation device is the perfect step between a shaken screening system and a bioreactor. Using this system, the performance of different strains can be assessed using an identical protocol, or the effect of selected upstream process parameters on protein production can be evaluated.
In-house 1 L and 10 L bioreactors are used to confirm the productivity levels of top-performing clones, and to generate material for purification. Our fermentation specialists have developed highly transferable and scalable protocols for both methanol-induced and methanol-free processes.
Our DSP scientists can develop a protein purification protocol to provide you with a non-GMP sample in a buffer of your choice. According to your requirements, we will also take downstream processing one step further providing full protein purification process development for a robust scalable process.
Feasibility studies provide a fast and cost-efficient way to check how well your protein can be expressed in Pichia pastoris. They allow you to obtain an early indication of success and a small sample for testing before committing to a larger development project. This reduces risk and supports informed decision-making at an early stage.
We create non-optimized stable strains using a few molecular tools from our UNLOCK PICHIA toolbox, test expression at microscale, and if needed, scale up to a 1 L bioreactor for a small protein sample, which can be purified if desired.
Feasibility studies are designed to be fast-track projects. Strain development can take as little as 4 weeks, followed by 2–5 weeks for cultivation and purification, depending on requirements.
VALIDOGEN's expert team is happy to discuss your project and help find the right solution. Send any project related requests to project@validogen.com, directly to our scientists, or use our contact form.
