Assessment of expression potential

Straightforward determination of expression potential & rapid production of protein samples
You instantly need a few mgs of recombinant protein?

Expression in other systems is troublesome or failed?

Expression in commercially available yeasts was not satisfying?

Are you searching for a powerful expression system suitable for industrial protein production?

In a lean feasibility study we will elucidate the expression potential of your recombinant protein in minimum time. Starting from an optimized synthetic gene sequence, results will be available within 3 - 4 weeks including cloning, transformation and screening giving you an immediate answer for a reliable go/no-go decision. Positive candidates from micro-scale screening can be directly transferred to bioreactor cultivation enabling both the production of initial protein samples as well as further assessment of the potential for improvement.

After positive assessment we provide yield optimization by applying VALIDOGEN's comprehensive production strain development.

Key benefits

  • Fast assessment of expression feasibility and potential for further improvement
  • Immediate answers for a reliable go/no-go decision
  • Results from micro-scale screening transferable to bioreactor cultivation
  • Rapid generation of protein samples and protein production strains


  • Small scale production of non-GMP protein samples
  • Glycerol stocks of expression strains
  • Comprehensive report


  • Methanol-induced and methanol-free processes
  • Codon optimization for maximum protein yields
  • Customized screening size applying a subset of VALIDOGEN's AOX1 promoter library
  • Helper factor co-expression
  • High-throughput cultivation and screening in 96-deep well plates
  • Cultivation of most productive clones in 1 l or 7.5 l bioreactors for rapid protein production

Further services

UNLOCK PICHIA - Pichia pastoris protein expression system

Be competitive with high-quality VALIDOGEN Pichia proteins, high-productivity strains & robust and scalable bioprocesses

Time-saving protein expression studies

Assessing the potential - VALIDOGEN's streamlined feasibility studies



Comprehensive strain development

Unlock the potential by applying VALIDOGEN's broad Pichia toolbox



Bioprocess development

Straightforward fermentation development, optimization and characterization services

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization

Cell banking                    

Preparation and characterization of research cell banks

Enzyme engineering

High-throughput engineering and screening of enzyme variants
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