Yield optimization through comprehensive production strain development

Apply VALIDOGEN's entire protein production toolbox UNLOCK PICHIA to increase product quality and enhance product yields through comprehensive production strain development

You need to maximize your yield in Pichia to be competitive?

Are you seeking to improve product quality of your protein?

Are you in search of high performance expression strains efficiently expressing your protein suitable for commercial protein production?

According to your requirements we will boost the expression of your protein by applying VALIDOGEN's full protein production toolbox known as UNLOCK PICHIA comprising an unparalleled spectrum of expression tools and ingenious expression strategies. Advantageously, at the same time protein quality aspects will be improved. VALIDOGEN's yield optimization & strain development program is based on the application of a higher number of genetic elements from UNLOCK PICHIA facilitating optimized fine-tuning of gene expression and enhanced production of high-quality proteins. We will rapidly identify high performance production strains secreting your protein with highest product yields and quality by the application of VALIDOGEN's combinatorial microscale cultivation & screening regime. We further unlock Pichia´s potential by applying robust and highly proven cultivation procedures through VALIDOGEN's straightforward process development and optimization program.

Applicable for

  • Following confirmation of recombinant protein expression in a feasibility study we will move on to optimize production of your protein
  • Enhancement of product yields & quality
  • Development of commercially viable protein expression strains

Key benefits

  • Accelerated generation of high-performance expression strains optimized for the production of commercial protein products
  • Enhancement of production levels & product quality
  • Fast and straightforward non-GMP production of protein samples
  • Reduced downstream processing efforts



  • Protein in cell-free culture supernatants or as purified sample
  • Glycerol-stocks of expression strains
  • Full documentation to support your regulatory filings
  • Comprehensive report



  • Codon optimization for maximization of protein yield
  • Methanol-induced and methanol-free processing
  • Extended screening size: combinatorial screening of several thousand clones by simultaneous application of a diverse set of AOX1 promoter variants from VALIDOGEN's AOX1 promoter library
  • Helper factor co-expression for enhancement of protein production
  • High-throughput cultivation enables reliable identification of high-productivity clones in 96-well format
  • For assessment of up-scale potential and protein production the most promising candidates are subsequently taken from 96-deep well plate screening into 1 l or 7.5 l bioreactors


Further Services

UNLOCK PICHIA - Pichia pastoris protein expression system

Be competitive with high-quality VALIDOGEN Pichia proteins, high-productivity strains & robust and scalable bioprocesses

Time-saving protein expression studies

Assessing the potential - VALIDOGEN's streamlined feasibility studies



Comprehensive strain development

Unlock the potential by applying VALIDOGEN's broad Pichia toolbox



Bioprocess development

Straightforward fermentation development, optimization and characterization services

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization

Cell banking                    

Preparation and characterization of research cell banks

Enzyme engineering

High-throughput engineering and screening of enzyme variants
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