Yield optimization through comprehensive production strain development
Apply VALIDOGEN's entire protein production toolbox UNLOCK PICHIA to increase product quality and enhance product yields through comprehensive production strain development
You need to maximize your yield in Pichia to be competitive?
Are you seeking to improve product quality of your protein?
Are you in search of high performance expression strains efficiently expressing your protein suitable for commercial protein production?
According to your requirements we will boost the expression of your protein by applying VALIDOGEN's full protein production toolbox known as UNLOCK PICHIA comprising an unparalleled spectrum of expression tools and ingenious expression strategies. Advantageously, at the same time protein quality aspects will be improved. VALIDOGEN's yield optimization & strain development program is based on the application of a higher number of genetic elements from UNLOCK PICHIA facilitating optimized fine-tuning of gene expression and enhanced production of high-quality proteins. We will rapidly identify high performance production strains secreting your protein with highest product yields and quality by the application of VALIDOGEN's combinatorial microscale cultivation & screening regime. We further unlock Pichia´s potential by applying robust and highly proven cultivation procedures through VALIDOGEN's straightforward process development and optimization program.
Following confirmation of recombinant protein expression in a feasibility study we will move on to optimize production of your protein
Enhancement of product yields & quality
Development of commercially viable protein expression strains
Accelerated generation of high-performance expression strains optimized for the production of commercial protein products
Enhancement of production levels & product quality
Fast and straightforward non-GMP production of protein samples
Reduced downstream processing efforts
Protein in cell-free culture supernatants or as purified sample
Glycerol-stocks of expression strains
Full documentation to support your regulatory filings
Codon optimization for maximization of protein yield
Methanol-induced and methanol-free processing
Extended screening size: combinatorial screening of several thousand clones by simultaneous application of a diverse set of AOX1 promoter variants from VALIDOGEN's AOX1 promoter library
Helper factor co-expression for enhancement of protein production
High-throughput cultivation enables reliable identification of high-productivity clones in 96-well format
For assessment of up-scale potential and protein production the most promising candidates are subsequently taken from 96-deep well plate screening into 1 l or 7.5 l bioreactors
UNLOCK PICHIA - Pichia pastoris protein expression system
Be competitive with high-quality VALIDOGEN Pichia proteins, high-productivity strains & robust and scalable bioprocesses