Downloads

Discover our latest articles and information around Pichia based protein expression

Company Information

UNLOCK PICHIA® Services

validogen-unlock-pichia-services.pdf Download

UNLOCK PICHIA® Technology

validogen-unlock-pichia-technology.pdf Download

UNLOCK PICHIA® Track Record and Company profile

validogen-track-record-and-company-profile.pdf Download

UNLOCK PICHIA® Methanol-free protein production technology

Methanol-free-protein-expression-pichia Download

White Paper

Boosted expression of different VHH antibody formats through application of VALIDOGEN’s UNLOCK PICHIA toolbox

We conducted a series of case studies, demonstrating the yield-enhancing effects of applying different toolbox elements of VALIDOGEN's Pichia pastoris protein expression system known as UNLOCK PICHIA®.

vhh-protein-expression-case-study-white-paper-validogen.pdf Download

High-level methanol-free phytase production in Pichia pastoris

methanol-free-protein-production-white-paper-validogen.pdf Download

Case Studies

Case study on methanol-free VHH production in Pichia pastoris

Maximizing product titer and space-time yield

Explore our latest two case studies showcasing the power of our methanol-free Pichia protein production technology. By leveraging our unique UNLOCK PICHIA® platform, we have developed optimized, scalable solutions for high-yield methanol-free protein production suitable for commercial manufacturing

methanol-free-VHH-production-in-pichia-case-study-unlock-pichia.pdf Download

Case study on methanol-induced VHH production in Pichia pastoris

Case Study III: Learn how VALIDOGEN's extensive strain and process development achieved an impressive 31 g/L productivity for production of a bivalent VHH.

methanol-induced-vhh-production-pichia-pastoris-validogen-case-study.pdf Download

Editorials

Pichia Protein Production

Check out our latest article on Pichia protein production, featured in the European Biotechnology Autumn Edition, Vol. 23, 2024.

Recombinant protein production in Pichia typically relies on AOX1 promoter-driven expression using methanol for induction. However, some companies avoid methanol due to safety concerns or operational demands. VALIDOGEN's unique AOX1 promoter variants enable methanol-free protein production at high space-time yields, offering advantages beyond safety. Latest case studies underscore the strength of this technology.

Pichia protein production - European Biotechnology 2024 Download

Further Information

Technology Overview
UNLOCK PICHIA® Technology
The Advantages of Pichia pastoris