UNLOCK PICHIA® Services
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Discover our latest articles and information around Pichia based protein expression
Company Information
UNLOCK PICHIA® Technology
pichia-pastoris-expression-system-unlock-pichia.pdf
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UNLOCK PICHIA® Track Record and Company profile
track-record-and-company-profile-validogen.pdf
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UNLOCK PICHIA® Methanol-free protein production technology
Learn how our UNLOCK PICHIA® platform optimizes protein production without methanol.
Methanol-free-pichia-protein-expression-system.pdf
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White Paper
Boosted expression of different VHH antibody formats through application of VALIDOGEN’s UNLOCK PICHIA toolbox
We conducted a series of case studies, demonstrating the yield-enhancing effects of applying different toolbox elements of VALIDOGEN's Pichia pastoris protein expression system known as UNLOCK PICHIA®.
vhh-protein-expression-case-study-white-paper-validogen.pdf
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Methanol-free phytase production in Pichia pastoris
methanol-free-protein-production-white-paper-validogen.pdf
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Case Studies
Case study on methanol-free VHH production in Pichia pastoris
Explore VHH case study I an II showcasing the power of our methanol-free Pichia protein production technology. By leveraging our unique UNLOCK PICHIA® platform, we have developed optimized, scalable solutions for high-yield methanol-free VHH production suitable for commercial manufacturing
Methanol-Free VHH Expression in Pichia pastoris: Maximizing Product Titer and Space-Time Yield
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Case study on methanol-induced VHH production in Pichia pastoris
Learn how VALIDOGEN's extensive strain and process development achieved an impressive 31 g/L productivity for production of a bivalent VHH.
VHH expression in Pichia pastoris - Case study - Methanol-induced protein production
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Summary - Case study on methanol-induced and methanol-free VHH production in Pichia pastoris
EXPLORE OUR LATEST CASE STUDIES ON VHH PRODUCTION - Summary of Case Study I, II, II
vhh-expression-pichia-case-study-validogen-unlockpichia-2025.pdf
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Editorials
Unlocking Pichia for Industry
Check out our latest article Unlocking Pichia for Industry, featured in the European Biotechnology Magazine Summer Edition, Vol. 24, 2025.
Discover how our proprietary UNLOCK PICHIA® system unlocks the full potential of Pichia pastoris — from methanol-free processes to scalable expression at 100,000 L.
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Pichia Protein Production
Check out our latest article on Pichia protein production, featured in the European Biotechnology Autumn Edition, Vol. 23, 2024.
Recombinant protein production in Pichia typically relies on AOX1 promoter-driven expression using methanol for induction. However, some companies avoid methanol due to safety concerns or operational demands. VALIDOGEN's unique AOX1 promoter variants enable methanol-free protein production at high space-time yields, offering advantages beyond safety. Latest case studies underscore the strength of this technology.
Pichia protein production - Article in European Biotechnology 2024
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