Rapid and reliable generation & identification of high-level Pichia pastoris production strains matching protein quantity and quality attributes
, a broad diversity of genetic arrangements and expression profiles is generated by applying promoter variants simultaneously in pools of expression constructs. These harbor the gene of interest as well as genes encoding for different auxiliary proteins, all under the control of numerous variants from our exclusive promoter library. In this setting, protein production in Pichia pastoris
is boosted significantly.VALIDOGEN
established a solid high-throughput protocol for comparative microscale cultivation and screening performed in 96-deep well plates. Thousands of clones can be screened in minimum time and most promising clones will be reliably identified already at microscale.
Moreover, VALIDOGEN´s established screening protocol facilitates the direct transfer of selected clones from 96-deep well plates into 1 l bioreactors for confirmation of effective productivity making laborious intermediate steps for clone comparison obsolete.
This gives VALIDOGEN the ability to rapidly and reliably identify production strains with highest product yields.
- Optimized cultivation and screening regime
- Thousands of clones screened in minimum time
- Highly reproducible performance data in micro-culture format
- Fast and reliable identification of high-performance production clones
- Results of microplate screening transferable to larger scales
Further elements of UNLOCK PICHIA - VALIDOGEN's Pichia pastoris protein production toolbox