Methanol-free expression in Pichia pastoris/Komagataella phaffii is emerging as a high-performance alternative to traditional AOX1 promoter-driven systems, offering strong, time-controlled protein induction without the safety and handling concerns of methanol. VALIDOGEN’s recent case studies demonstrate how optimized MeOH-free processes can deliver high space-time yields and product titers of up to 19 g/L, making them a compelling option for biopharma and industrial protein production.