AOX1 promoter libraries for Pichia protein production

Exclusive AOX1 promoter libraries for fine-tuned methanol-inducible or methanol-free protein production

Maximization of protein production -

1^st generation methanol-inducible AOX1 promotor variants

Key benefits

Productivity records of 35 g/L of secreted protein in supernatant for methanol-induced protein expression
Large number of exclusive AOX1 promoter variants for proper fine-tuning of gene expression
Robust processes with low cost of goods
Selection of perfect match of the respective promoter variant and your target gene

VALIDOGEN's highly proven 1^st generation promoter variants form the core of VALIDOGEN´s cutting-edge Pichia pastoris toolbox known as UNLOCK PICHIA enabling fine-tuned high level protein expression of your protein.

These methanol-inducible promoter variants enable the fine tuning of gene expression by selecting the perfect match of promoter/target gene combinations. The technology can further be used to enable cells to provide expression enhancing factors in a well dosed manner and just in time during expression of a target protein. Both yield and quality of the target protein are thus maximized.

VALIDOGEN's unique methanol-free Pichia protein production system -

2^nd generation AOX1 promoter variants

Key benefits

Productivity records of 20 g/l of secreted protein in supernatant for methanol-free protein expression in Pichia
Improved process safety by abolishing toxic and explosive methanol
Robust processes with low cost of goods
Decreased demand for cooling and aeration
Glycerol or glucose as the sole carbon source
Potential to significantly reduce process times

VALIDOGEN's new 2^nd generation AOX1 promoter variants allow for safe and economically viable protein production in glycerol- or glucose-fed processes fully devoid of methanol.

Apart from improving safety by abolishing toxic and explosive methanol as a substrate, major advantages of this technology are a reduced oxygen consumption and therefore a significantly reduced heat production and cooling effort in bioreactor cultivations. Additionally, there is a high potential to reduce process time and cost of goods.

Click here for our latest WHITE PAPER entitled "High level methanol-free phytase production in Pichia pastoris"

Further elements of UNLOCK PICHIA - VALIDOGEN's Pichia pastoris protein production technology

Exclusive AOX1 promoter libraries

UNLOCK PICHIA - discover the broadest Pichia pastoris protein production toolbox

MeOH-inducible and MeOH-free AOX1 promoter libraries

Diverse platform strains

Proprietary helper factors

High-throughput combinatorial screening

Glycoengineering tools

Half-life extension

News

Meet us at

Spotlight on VALIDOGEN’s strain and process development program

Press

Press center

Downloads

Company folder and brochures