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Exclusive AOX1 promoter libraries

 

Exclusive AOX1 promoter libraries for fine-tuned methanol-inducible or methanol-free protein production
Maximization of protein production -
1st generation methanol-inducible AOX1 promotor variants

Key benefits

  • Productivity records of 35 g/L of secreted protein in supernatant for methanol-induced protein expression
  • Large number of exclusive AOX1 promoter variants for proper fine-tuning of gene expression
  • Robust processes with low cost of goods
  • Selection of perfect match of the respective promoter variant and your target gene

 

VALIDOGEN's highly proven 1st generation promoter variants form the core of VALIDOGEN´s cutting-edge Pichia pastoris toolbox known as UNLOCK PICHIA enabling fine-tuned high level protein expression of your protein.

These methanol-inducible promoter variants enable the fine tuning of gene expression by selecting the perfect match of promoter/target gene combinations. The technology can further be used to enable cells to provide expression enhancing factors in a well dosed manner and just in time during expression of a target protein. Both yield and quality of the target protein are thus maximized.





 

VALIDOGEN's unique methanol-free Pichia protein production system -
2nd generation AOX1 promoter variants
 

Key benefits

  • Productivity records of 20 g/l of secreted protein in supernatant for methanol-free protein expression in Pichia
  • Improved process safety by abolishing toxic and explosive methanol
  • Robust processes with low cost of goods
  • Decreased demand for cooling and aeration
  • Glycerol or glucose as the sole carbon source
  • Potential to significantly reduce process times

 

VALIDOGEN's new 2nd generation AOX1 promoter variants allow for safe and economically viable protein production in glycerol- or glucose-fed processes fully devoid of methanol.

Apart from improving safety by abolishing toxic and explosive methanol as a substrate, major advantages of this technology are a reduced oxygen consumption and therefore a significantly reduced heat production and cooling effort in bioreactor cultivations. Additionally, there is a high potential to reduce process time and cost of goods.

Click here for our latest WHITE PAPER entitled "High level methanol-free phytase production in Pichia pastoris"

 

Further elements of UNLOCK PICHIA - VALIDOGEN's Pichia pastoris protein production technology

UNLOCK PICHIA - discover the broadest Pichia pastoris protein production toolbox

Unparalleled spectrum of exclusive expression tools and ingenious expression strategies
01

MeOH-inducible and MeOH-free AOX1 promoter libraries

Maximize productivity by fine-tuning of protein expression
Details
02

Diverse platform strains

Meet your needs for various applications
Details
03

Proprietary helper factors

Enhancement of product yield and quality – timely adjusted for maximum output
Details
04

High-throughput combinatorial screening

Rapid and reliable generation & identification of high-level production strains matching protein quantity and quality attributes
Details
05

Glycoengineering tools

Enhanced glycoprotein production – UNLOCK PICHIA combined with Pichia GlycoSwitch®
Details
06

Half-life extension

Albumin fusion proteins - biopharmaceuticals with increased half-life
Details

News

Spotlight on high-yield VHH expression using UNLOCK PICHIA

Benefit from the economic advantages of enhanced VHH production arising from ...
Details

VTU Technology is now VALIDOGEN

VTU Technology is now VALIDOGEN
Details

Downloads

Company folder and brochures

All downloads
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