In order to provide you with a highly pure sample of your protein in short time, we will set up a purification process that will yield milligram to gram quantities of pure protein sample from a Pichia culture supernatant. Based on our experience and taking into account the properties of your protein, we will scout a set of promising methods to come up with a purification strategy focusing on speed and purity. Purification methods applied are chosen from standard chromatography techniques such as ion exchange or hydrophobic interaction as well as affinity chromatography in combination with ultrafiltration steps.
According to your requirements, we will also take downstream processing one step further providing full process development for a robust scalable process. In close coordination with our customers, we will evaluate capacities, maximize yield and purity, establish clearance of impurities and understand how subsequent steps are interlinked. Comprehensive documentation and support guarantee neat transfer of the optimized downstream process to your production site.
All DSP activities are evidently accompanied by analytical characterization of process intermediates and purified product. Established in house methods include 1- and 2D-PAGE, capillary electrophoresis, HPLC, Western Blot, ELISA as well as methods to quantify process related impurities, including HCP and host cell DNA.