Enzyme engineering services

VALIDOGEN's high-throughput enzyme engineering service for the improvement of the properties of your enzyme
Supporting you in product innovation, VALIDOGEN offers fast and reliable engineering and screening of improved enzyme variants.

The key advantage of using UNLOCK PICHIA - VALIDOGEN's Pichia system for the generation of enzyme variants is that engineering is performed in the final production host. Therefore, potential bottlenecks in changing the host system from primary screen to final enzyme production are eliminated, thus accelerating the commercialization of your improved enzyme. In addition, VALIDOGEN's established high-throughput microscale cultivation and screening protocols facilitate the rapid identification of improved protein variants with specific properties. Application of UNLOCK PICHIA  ensures production of high-value enzymes at lower manufacturing costs.

Next to classical objectives like increasing activity towards a specific substrate, any kind of increased stability upon incubation at e.g. varying temperatures, differing pH-values or diverse reaction matrices has been successfully achieved.

Libraries of gene variants are created by error-prone PCR and/or site directed mutagenesis potentially supported by molecular modeling. The formation of single copy integrations is favored by using appropriate amounts of linearized DNA paired with the ideal selection pressure in order to allow for highest comparability in screenings. Using VALIDOGEN's established 96-deep-well plate cultivation for homogeneous growth of individual transformants, high-throughput screening of thousands of gene variants in parallel is carried out using spectrophotometric or fluorometric activity analysis. In addition, a pre-screening on solid media to eventually cultivate only a selected set of transformants can be envisaged.

Key benefits

  • High-throughput-screening for engineered variants using a eukaryotic production system
  • Engineering for improved variants performed in final production host
  • VALIDOGEN's established high-throughput microscale cultivation and screening protocols facilitate the fast and reliable identification of protein variants with desired enhanced properties
  • Short timelines and high flexibility
  • Projects tailored to your needs



  • Samples of optimized protein variants
  • Production strains of engineered variants
  • Comprehensive report



  • Library generation using classical and tailored mutagenesis tools
  • Transformation of library into Pichia pastoris favoring single copy integration events
  • Cultivation using VALIDOGEN's established high-throughput microscale protocol
  • Screening based on liquid spectrophotometric or fluorometric assays
  • Pre-screening on solid media possible
Further Services

UNLOCK PICHIA - Pichia pastoris protein expression system

Be competitive with high-quality VALIDOGEN Pichia proteins, high-productivity strains & robust and scalable bioprocesses

Time-saving protein expression studies

Assessing the potential - VALIDOGEN's streamlined feasibility studies



Comprehensive strain development

Unlock the potential by applying VALIDOGEN's broad Pichia toolbox



Bioprocess development

Straightforward fermentation development, optimization and characterization services

Protein purification and DSP development

Protein purification and establishment of analytical procedures and product characterization

Cell banking                    

Preparation and characterization of research cell banks

Enzyme engineering

High-throughput engineering and screening of enzyme variants
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