x

P. pastoris - simple, fast, robust, productive and scalable

Pichia facts - be competitive with the most cost-effective eukaryotic protein expression system
Pichia pastoris (Komagataella phaffii) is an established, FDA approved, safe (GRAS) and highly competitive expression host with strong secretory capacities while secreting only low amounts of endogenous proteins. Today, a large number of recombinant protein products on the market (pharma and non-pharma applications) are produced in Pichia pastoris.


The yeast P. pastoris combines the advantages of prokaryotes, such as easy genetic manipulation and fast growth on inexpensive & chemically defined media with eukaryotic features such as a subcellular protein processing machinery needed for post-translational modification.


Yet another advantageous feature is Pichia´s ability to actively and effectively secrete recombinant target proteins into the culture supernatant with an exceptionally low amount of extracellular endogenous proteins furnishing a raw product with high purity. Thus, Pichia not only enables cultivation processes with high volumetric productivity but also simplifies downstream processes rendering manufacturing of recombinant proteins even more economically viable.

As compared to e.g. E. coli, no cell lysis and tedious isolation from a usually very crude lysate or refolding from inclusion bodies is required to obtain the target protein. At the same time the probability of unwanted enzymatic side activities is minimized.

In contrast to laborious, expensive and time-consuming mammalian cell line generation, generation of stable recombinant protein producing Pichia cell lines is straightforward. Pichia pastoris combines the speed and ease of highly efficient prokaryotic platforms and the secretion capabilities of mammalian systems reducing the effort for protein purification.

 

Key features

  • Eukaryotic protein expression system
  • FDA and EMA approved host for manufacturing of biopharmaceuticals
  • Straightforward and quick construction of stable cell lines
  • Advantages of a eukaryotic protein processing machinery facilitating protein folding and post-translational modification
  • Active product secretion into culture supernatant
  • Simplified recovery and protein purification
  • Pichia pastoris is ideally suited for the expression of complex proteins (not expressible in E.coli)
  • Intrinsically endotoxin and virus free
  • Robust expression simplifying cultivation and scale-up
  • Excellent scalability – scales of up to ~200.000 liters
  • Simple, chemically defined and animal-component-free media
Why UNLOCK PICHIA? Why VALIDOGEN´s Pichia pastoris protein production system?

Pichia pastoris – simple, fast and cost-effective eukaryotic protein expression

Be competitive with VALIDOGEN´s Pichia pastoris protein production toolbox
01

Pichia pastoris - Features

Why use Pichia pastoris as an expression host?
Details
02

UNLOCK PICHIA - Pichia pastoris protein production toolbox 

Unlock the potential - why use VALIDOGEN´s Pichia pastoris expression system?
Details

News

Spotlight on high-yield VHH expression using UNLOCK PICHIA

Benefit from the economic advantages of enhanced VHH production arising from ...
Details

VTU Technology is now VALIDOGEN

VTU Technology is now VALIDOGEN
Details

Downloads

Company folder and brochures

All downloads
Tablet Potrait-Mode gerade in Arbeit, bitte drehen Sie das Tablet in den Landscape-Mode.