Spotlight on VALIDOGEN’s strain and process development program

1. VALIDOGEN`s advanced protein production strain development supported by microbioreactors

VALIDOGEN has recently broadened its strain development capabilities with a microbioreactor system serving as a time- and resource-saving tool for Pichia pastoris strain development. With the company‘s established high-throughput protocols for comparative microscale cultivation in 96-deep well plates, thousands of clones can be screened in minimum time and most promising clones will be reliably identified already at microscale. 

However, in cases where a larger number of similarly promising clones are identified in 96-deep well plate based microscale screening, microbioreactor cultivations can be performed to rapidly compare these clones under more tightly controlled conditions as an intermediate step to narrow down the number of promising clones or already select the final producer clone(s). A confirmation of strain performance is then conducted in larger bioreactors. 

Therefore, microbioreactor cultivations represent a powerful support for clone selection during early upstream development in microliter scale.

2. VALIDOGEN's bioreactor cultivation process optimization workflows supported by microbioreactors

In addition to the benefits for VALIDOGEN`s Pichia strain development procedures, microbioreactors represent an appealing alternative to larger lab-scale bioreactors for cultivation process development including Design of Experiment (DoE) programs. While results are conclusive for bioreactors, the small reactor volumes and shortened cultivation time as well as the fact that a number of process parameters (such as temperature, pH and feed) can be efficiently controlled, render microbioreactors a powerful cultivation process development tool. This facilitates simultaneous cultivations with a variety of experimental conditions to be carried out in a time- and resource saving manner.