Pichia protein expression toolbox
UNLOCK PICHIA® for maximized productivity
UNLOCK PICHIA® Advantages
Exclusive AOX1 promoter libraries
Our unique promoter library based on the highly utilized AOX1 promoter allows for fine-tuning of protein expression. This includes inducible control with methanol as well as methanol-free expression, with the latter surpassing the abilities of traditionally used constitutive promoters. With a broad range of promoter characteristics developed, we will find the right promoter for your protein.
Set of advanced platform strains
Our suite of platform strains has been developed to enhance productivity and cell viability under a multitude of conditions. In addition, strains optimized for processes without methanol, and auxotrophic strains without antibiotic resistance markers (ARMs-free) allow us to find the right strain for your application.
Expression-enhancing helper factors
Co-transformation of our expression-enhancing helper factors allows for targeted de-bottlenecking at every step of intracellular protein processing. Transcription factors, chaperones, membrane proteins, and more promote the secretory pathway to aid the passage of your target protein.
Novel secretion signals
In addition to the industry standard α-mating factor (MF) secretion signal from S. cerevisiae, at VALIDOGEN we apply novel secretion signals and strategies to promote the passage of your protein from cell to supernatant. Our novel signals have been shown to repeatedly outperform the α-mating factor signal peptide for a variety of target proteins.
Glycoprotein engineering tools
VALIDOGEN’s UNLOCK PICHIA® can be combined with Pichia GlycoSwitch® technology to generate unique expression strains for the production of glycoproteins with human-like glycosylation patterns. This unique combination of technologies acting synergistically leads to a high-performance platform for the production of recombinant glycoproteins with superior product yields and uniform Man5 or other human-like glycoforms.
Predictive expression screening
Combinatorial evaluation of our UNLOCK PICHIA® tools is paired with a high-throughput screening system to quickly and reliably identify the highest-performing clones at microscale. As an intermediary step, microbioreactor cultivations can be performed to rapidly compare clones under more tightly controlled conditions. This can narrow down the number of promising clones and lead to final strain selection. A confirmation of strain performance is then conducted in larger bioreactors.
