RAFT® 13 - Recent Advances In Fermentation Technology, 27-30 October, Ft. Myers, FL16.09.2019
VALIDOGEN presenting UNLOCK PICHIA tools at RAFT® 13, October 27-30, 2019
Dr. Roland Weis, VALIDOGEN’s Head of Operations, will contribute to the ‘Cell factory design and engineering’ session of the upcoming RAFT® 13 - Recent Advances in Fermentation Technology (Sunday, October 27, 2019 2:00 PM - 4:30 PM) with his presentation on “UNLOCK PICHIA - a toolbox to maximize productivity through diversity”.
Novel product classes and current trends in biopharma production ask for versatile and yet robust expression systems. VALIDOGEN’s answer is a yield-enhancing protein production toolbox known as UNLOCK PICHIA enabling fine-tuning of protein expression by its diversity of molecular tools and expression strategies for Pichia. Continuous expansion & improvement of its technology platform facilitates the targeted debottlenecking of protein expression all the way from transcription and translation to translocation, protein folding and secretion thereby facilitating the expression of more complex proteins without compromising product quality. Selection of best-performing clones from strain engineering approaches in microscale, i.e. 96-deep-well plates, is directly followed by cultivation in benchtop-bioreactors at scales of 0.75L- and 5L-working volumes to reconfirm pre-set ranking of strains and at the same time gather information on growth and production behavior. Subsequently, bioreactor process optimization including scouting of classical parameters as e.g. pH-value, feeding regime and interplay of biomass level and feeding allows for maximizing yield of the target protein.