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The broadest Pichia protein production toolbox

Learn more about VTU´s service portfolio

TODAY'S FEATURE:

Assessing the potential -
VTU´s streamlined feasibility studies

Straightforward determination of expression potential & rapid production of protein samples

You instantly need a few mgs of recombinant protein?

Expression in other systems is troublesome or failed?

Expression in commercially available yeasts was not satisfying?

Are you searching for a powerful expression system suitable for industrial protein production?


In a lean feasibility study we will elucidate the expression potential of your recombinant protein in minimum time. Starting from an optimized synthetic gene sequence, results will be available within 3 to 4 weeks including cloning, transformation and screening giving you an immediate answer for a reliable go/no-go decision. Positive candidates from micro-scale screening can be directly transferred to bioreactor cultivation enabling both the production of initial protein samples as well as further assessment of the potential for improvement.

After positive assessment we provide yield optimization by applying
VTU´s comprehensive production strain development.

Key benefits

  • Customized screening size for fast assessment of expression feasibility
  • Immediate answers for a reliable go/no-go decision
  • Identification of potential for further improvement
  • Results from micro-scale screening transferable to bioreactor cultivation
  • Rapid generation of protein samples and protein production strains


Technology

  • Exclusive AOX1 promoter variants
  • Methanol-induced and methanol-free processes
  • Codon optimization for maximum protein yields
  • Helper factor co-expression
  • High-throughput cultivation and screening in 96-deep well plates
  • Cultivation of most productive clones in 1 L or 7.5 L bioreactors for rapid protein production


Learn more about VTU' s entire service portfolio.

Talk to VTU´s protein expression experts at one of the following upcoming events:

· 6th Halle Conference on Recombinant Proteins 2018, March 8 - 9, Halle/Salle, Germany

Iskandar Dib will be giving a presentation on March 8 at 12 am in session 2 "New Expression Technologies": Unlock Pichia - novel strategies and molecular tools to enhance protein expression

· BIO-Europe Spring 2018, March 12 - 14, Amsterdam, The Netherlands

  • BioProcess International Conference & Exhibition West 2018, March 19 - 22, San Francisco, US

VTU´s Pichia protein production toolbox
- Exclusive library of promoter variants
- Diverse platform strains
- Proprietary set of helper factors
- High-throughput combinatorial screening
- Glycoengineering tools
- Half-life extension strategies

VTU Technology GmbH
Parkring 18, 8074 Raaba-Grambach, Austria
office.technology@vtu.com

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