Key to success - the targeted application of VALIDOGEN's full toolbox
The UNLOCK PICHIA promoter libraries include high productivity methanol-inducible and methanol-free promoter variants, the latter for improved process safety and economy. The libraries allow for more effective fine-tuning of gene expression and significant productivity boost through application of an increased number of promoter variants transformed simultaneously as a pool. This approach is combined with the targeted application of VALIDOGEN's broad technology platform by identification of optimum combinations of different elements of the UNLOCK PICHIA toolbox such as strain background, helper factors, secretion signals or production regimes further boosting productivity.
Key to success - reliable identification of high-performance strains in microscale and microbioreactors
In addition to VALIDOGEN's high-throughput cultivation and screening regime, which allows rapid and reliable identification of high-level production strains in microscale, we have implemented microbioreactors enabling the assessment of the performance of selected strains from microscale screening under more tightly controlled cultivation conditions further leveraging the benefits of UNLOCK PICHIA. Thereby additional comparative data can be obtained supporting the selection of strains to be transferred to larger lab bioreactors.
Key to success - UNLOCK PICHIA boosting Pichia GlycoSwitch®
This unique combination of both technologies acting synergistically results in a powerful platform for the production of recombinant glycoproteins with superior product yields and uniform GlcNAc2Man5 or other human-like glycoforms.
Key to success - speed & efficiency allow for quick feasibility studies followed by comprehensive production strain generation
Feasibility studies are designed to elucidate the optimum combinations of genetic elements for a given protein of interest. Building on these findings, a highly efficient production strain is then developed by fully applying VALIDOGEN’s UNLOCK PICHIA toolbox thereby leveraging the full potential of the technology.