VALIDOGEN presenting advanced protein production tools at RPP conference in Crete, April 24-27, 2019
Dr. Roland Weis, VALIDOGEN’s Head of Operations, will contribute to the ‘Recombinant protein production processes at industrial scale’ session of the upcoming RPP10 conference on Day 3 (11.20 - 11.40 a.m., April 26) with his presentation on ‘Productivity through diversity - a protein production toolbox to UNLOCK PICHIA’.
“Novel product classes and current trends in biopharma production ask for versatile and yet robust expression systems. Our answer is a yield-enhancing protein production toolbox, ‘UNLOCK PICHIA’, that enables fine-tuning of protein expression by its diversity of molecular tools and expression strategies for Pichia,” Roland Weis explained.
His presentation will show how VALIDOGEN’s Pichia toolbox including an exclusive AOX1-promoter library (with and without methanol induction) leverages gene/protein-customized expression which translates into high-level production strains already identified from high-throughput cultivation and screening in microscale format.
Due to this robust deep-well plate cultivation method, rapid and most importantly reliable transfer to bioreactors and further scale-up is ensured, which is manifested by production campaigns in up to 100,000 L scale with a strain as well as a cultivation process developed with VALIDOGEN.
Clearing expression bottlenecks
Continuous expansion and improvement of its technology platform facilitates the targeted debottlenecking of protein expression all the way from transcription and translation to translocation, protein folding and secretion, thereby facilitating the expression of more complex proteins without compromising product quality.
Dr. Weis will share results from showcase productions as well as dedicated industrial projects, spanning the fields of biopharma and diagnostic products over industrial enzymes and enzyme engineering via directed evolution.