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VALIDOGEN - News
Pichia pastoris protein expression excellence
  • Microbioreactors supporting microscale Pichia strain selection and bioreactor cultivation process development
  • Watch the latest presentation on VALIDOGEN's upstream development workflows enhanced by the integration of microbioreactors
  • VALIDOGEN is hiring - "Research Scientist / R&D Associate, Molecular Biotechnology (m/f/d)"
Microbioreactors supporting microscale Pichia strain selection and bioreactor cultivation process development

VALIDOGEN has recently implemented microbioreactors to further advance the benefits of its UNLOCK PICHIA technology platform.

Microbioreactors are used as an intermediate step to support
1. VALIDOGEN's high-throughput microscale strain development and selection procedures
2. VALIDOGEN's bioreactor cultivation process optimization workflows


Key benefits of microbioreactors for VALIDOGEN's strain selection and cultivation process development
  • Time- and resource-saving intermediate step between microscale and bioreactor cultivation
  • Intermediate step to narrow down the number of promising clones identified in microscale screening
  • Tightly controlled bioreactor-like conditions (methanol-induced and methanol-free production)
  • Predictive of bioreactor cultivations
  • Enables simultaneous cultivations with a variety of experimental conditions (DoE)
1. VALIDOGEN`s advanced protein production strain development supported by microbioreactors
 
VALIDOGEN has recently broadened its strain development capabilities with a microbioreactor system serving as a time- and resource-saving tool for Pichia pastoris strain development. With the company‘s established high-throughput protocols for comparative microscale cultivation in 96-deep well plates, thousands of clones can be screened in minimum time and most promising clones will be reliably identified already at microscale.

However, in cases where a larger number of similarly promising clones are identified in 96-deep well plate based microscale screening, microbioreactor cultivations can be performed to rapidly compare these clones under more tightly controlled conditions as an intermediate step to narrow down the number of promising clones or already select the final producer clone(s). A confirmation of strain performance is then conducted in larger bioreactors.
 
Therefore, microbioreactor cultivations represent a powerful support for clone selection during early upstream development in microliter scale. 

Key benefits of VALIDOGEN`s optimized microscale cultivation and screening procedure
  • Thousands of clones screened in parallel in minimum time
  • Fast and reliable identification of high-performance production clones
  • Results of microscale screening transferable to larger scales
2. VALIDOGEN's bioreactor cultivation process optimization workflows supported by microbioreactors
 
In addition to the benefits for VALIDOGEN`s Pichia strain development procedures, microbioreactors represent an appealing alternative to larger lab-scale bioreactors for cultivation process development including Design of Experiment (DoE) programs. While results are conclusive for bioreactors, the small reactor volumes and shortened cultivation time as well as the fact that a number of process parameters (such as temperature, pH and feed) can be efficiently controlled, render microbioreactors a powerful cultivation process development tool. This facilitates simultaneous cultivations with a variety of experimental conditions to be carried out in a time- and resource saving manner.

Case study demonstrating the integration of microbioreactors in bioreactor cultivation process development
In order to demonstrate the usefulness of microbioreactors in bioreactor cultivation process development, we performed a DoE based study with a Pichia pastoris strain producing a bivalent VHH. We performed a full factorial design comprising 16 microbioreactor runs (4 factors, 2 levels), each run was carried out in duplicate.

Investigated factors 
  • amount of N-source
  • pH value
  • feed composition
  • feed rate
Results and confirmation in lab-scale bioreactors
As a result of this study several parameter sets yielding enhanced productivity were found and interactions between factors depending on their respective levels were elucidated.
 
Lab-scale bioreactor cultivations applying these optimized conditions confirmed the findings from the microbioreactor DoE. With the time- and resource-saving application of microbioreactors in this study, a ~40% improvement in VHH yield in comparison to standard conditions was achieved, clearly demonstrating the usefulness of microbioreactors for bioreactor cultivation process development as well as the sound transferability of results from microbioreactors into lab-scale bioreactor.
Please contact us if you are interested to learn more about VALIDOGEN's strain development or any other service

Iskandar Dib - iskandar.dib@validogen.com
Thomas Purkarthofer - thomas.purkarthofer@validogen.com 
Watch the latest presentation on VALIDOGEN's upstream development workflows enhanced by the integration of microbioreactors
Aid Atlic, Senior Scientist Process Development at VALIDOGEN 
VALIDOGEN is hiring
"Research Scientist / R&D Associate, Molecular Biotechnology (m/f/d)"
Currently, we are looking for a highly motivated Research Scientist / R&D Associate with hands on experience in molecular biotechnology and yeast based protein expression (PhD level mandatory)
Full-time, immediately
Please visit our job posting for more information 
For more information please visit our website or contact us directly:
VALIDOGEN GmbH
Parkring 18, 8074 Raaba-Grambach, Austria
office@validogen.com, T: + 43 316 4009 4000
www.validogen.com
LinkedIn
Website
Email






This email was sent to e.trummer-goedl@validogen.com
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VALIDOGEN GmbH · Parkring 18 · Raaba-Grambach 8074 · Austria

Spotlight on microbioreactors supporting microscale Pichia protein production strain selection and bioreactor cultivation process development
View this email in your browser
VALIDOGEN - News
Pichia pastoris protein expression excellence
  • Microbioreactors supporting microscale Pichia strain selection and bioreactor cultivation process development
  • Watch the latest presentation on VALIDOGEN's upstream development workflows enhanced by the integration of microbioreactors
  • VALIDOGEN is hiring - "Research Scientist / R&D Associate, Molecular Biotechnology (m/f/d)"
Microbioreactors supporting microscale Pichia strain selection and bioreactor cultivation process development

VALIDOGEN has recently implemented microbioreactors to further advance the benefits of its UNLOCK PICHIA technology platform.

Microbioreactors are used as an intermediate step to support
1. VALIDOGEN's high-throughput microscale strain development and selection procedures
2. VALIDOGEN's bioreactor cultivation process optimization workflows


Key benefits of microbioreactors for VALIDOGEN's strain selection and cultivation process development
  • Time- and resource-saving intermediate step between microscale- and bioreactor cultivation
  • Intermediate step to narrow down the number of promising clones identified in microscale screening
  • Tightly controlled bioreactor-like conditions (methanol-induced and methanol-free production)
  • Predictive of bioreactor cultivations
  • Enables simultaneous cultivations with a variety of experimental conditions (DoE)
1. VALIDOGEN`s advanced protein production strain development supported by microbioreactors
 
VALIDOGEN has recently broadened its strain development capabilities with a microbioreactor system serving as a time- and resource-saving tool for Pichia pastoris strain development. With the company‘s established high-throughput protocols for comparative microscale cultivation in 96-deep well plates, thousands of clones can be screened in minimum time and most promising clones will be reliably identified already at microscale.

However, in cases where a larger number of similarly promising clones are identified in 96-deep well plate based microscale screening, microbioreactor cultivations can be performed to rapidly compare these clones under more tightly controlled conditions as an intermediate step to narrow down the number of promising clones or already select the final producer clone(s). A confirmation of strain performance is then conducted in larger bioreactors.
 
Therefore, microbioreactor cultivations represent a powerful support for clone selection during early upstream development in microliter scale. 

Key benefits of VALIDOGEN`s optimized microscale cultivation and screening procedure
  • Thousands of clones screened in parallel in minimum time
  • Fast and reliable identification of high-performance production clones
  • Results of microscale screening transferable to larger scales
2. VALIDOGEN's bioreactor cultivation process optimization workflows supported by microbioreactors
 
In addition to the benefits for VALIDOGEN`s Pichia strain development procedures, microbioreactors represent an appealing alternative to larger lab-scale bioreactors for cultivation process development including Design of Experiment (DoE) programs. While results are conclusive for bioreactors, the small reactor volumes and shortened cultivation time as well as the fact that a number of process parameters (such as temperature, pH and feed) can be efficiently controlled, render microbioreactors a powerful cultivation process development tool. This facilitates simultaneous cultivations with a variety of experimental conditions to be carried out in a time- and resource saving manner.

Case study demonstrating the integration of microbioreactors in bioreactor cultivation process development
In order to demonstrate the usefulness of microbioreactors in bioreactor cultivation process development, we performed a DoE based study with a Pichia pastoris strain producing a bivalent VHH. We performed a full factorial design comprising 16 microbioreactor runs (4 factors, 2 levels), each run was carried out in duplicate.

Investigated factors 
  • amount of N-source
  • pH value
  • feed composition
  • feed rate
Results and confirmation in lab-scale bioreactors
As a result of this study several parameter sets yielding enhanced productivity were found and interactions between factors depending on their respective levels were elucidated.
 
Lab-scale bioreactor cultivations applying these optimized conditions confirmed the findings from the microbioreactor DoE. With the time- and resource-saving application of microbioreactors in this study, a ~40% improvement in VHH yield in comparison to standard conditions was achieved, clearly demonstrating the usefulness of microbioreactors for bioreactor cultivation process development as well as the sound transferability of results from microbioreactors into lab-scale bioreactor.
Please contact us if you are interested to learn more about VALIDOGEN's strain development or any other service

Iskandar Dib - iskandar.dib@validogen.com
Thomas Purkarthofer - thomas.purkarthofer@validogen.com 
Watch the latest presentation on VALIDOGEN's upstream development workflows enhanced by the integration of microbioreactors
Aid Atlic, Senior Scientist Process Development at VALIDOGEN 
VALIDOGEN is hiring
"Research Scientist / R&D Associate, Molecular Biotechnology (m/f/d)"
Currently, we are looking for a highly motivated Research Scientist / R&D Associate with hands on experience in molecular biotechnology and yeast based protein expression (PhD level mandatory)
Full-time, immediately
Please visit our job posting for more information 
For more information please visit our website or contact us directly:
VALIDOGEN GmbH
Parkring 18, 8074 Raaba-Grambach, Austria
office@validogen.com, T: + 43 316 4009 4000
www.validogen.com
LinkedIn
Website
Email






This email was sent to e.trummer-goedl@validogen.com
why did I get this?    unsubscribe from this list    update subscription preferences
VALIDOGEN GmbH · Parkring 18 · Raaba-Grambach 8074 · Austria