This talk aims to illustrate how VALIDOGEN established strategies for maximization of space-time yield as well as total product titer for methanol-free protein production using UNLOCK PICHIA®.
VALIDOGEN’s unique AOX1 promoter variants that enable methanol-free protein production at multi-gram-per-liter titers offer several advantages that go far beyond mere safety concerns. Using methanol-free production combined with other elements of VALIDOGEN’s UNLOCK PICHIA toolbox, we established strategies to maximize space-time-yield and total product titer. Optimized methanol-free processes enable considerably shorter batch times and even continuous upstream processes, thus paving the way toward intensified processes for biopharma and precision fermentation.
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