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Don't miss latest insights into UNLOCK PICHIA technology developments at ESIB - PICHIA 2022 
VALIDOGEN to present at PICHIA 2022, November 15-17, Graz, Austria
Dr. Aid Atlic 
Senior Scientist Process Development

High-productivity methanol-free protein production
Day 2, November 16, 05.35 p.m

This talk aims to illustrate how VALIDOGEN established strategies for maximization of space-time yield as well as total product titer for methanol-free protein production using UNLOCK PICHIA®. 

The most widely used approach for recombinant protein production using Pichia pastoris as host is to express the gene of interest under control of AOX1 promoter applying methanol induction. However, many companies prefer not to use methanol due to safety concerns (e.g. for medical, food and feed products). To address this issue, VALIDOGEN has developed a set of AOX1 promoter variants that enable methanol-free protein production at multi-gram-per-liter titers. Methanol-free fermentation processes offer several advantages over those with methanol induction, such as low safety issues due to omittance of hazardous compounds, more economical production plant requirements and lower oxygen demand. Using components of VALIDOGEN’s UNLOCK PICHIA toolbox and process development methods, we established strategies for maximization of space-time yield as well as total product titer. Examples and case studies will be presented that show the applications of high-productivity methanol-free protein production processes with Pichia pastoris at various scales.
VALIDOGEN is part of PEPTALK 2023, January 16-20, San Diego, US
VALIDOGEN will be represented by Iskandar Dib and Roland Weis. If you are interested in meeting us there, we would be very happy to arrange a meeting. Just contact us directly. We look forward to connecting with you! 
Watch the latest presentation on VALIDOGEN's upstream development workflows enhanced by the integration of microbioreactors
UNLOCK PICHIA advantages 
  • Broadest Pichia platform: numerous expression tools & different strategies for the enhancement of production yields and product quality
  • Targeted debottlenecking for yield maximization
  • Secreted products - beneficial starting point for protein purification
  • Complete absence of methanol through VALIDOGEN´s methanol-free promoters
  • Tailor-made glycoproteins - UNLOCK PICHIA boosting Pichia GlycoSwitch®
  • Stable, robust and scalable processes for commercial manufacturing
For more information please visit our website or contact us directly:
Parkring 18, 8074 Raaba-Grambach, Austria, T: + 43 316 4009 4000

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VALIDOGEN GmbH · Parkring 18 · Raaba-Grambach 8074 · Austria