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This talk aims to illustrate how VALIDOGEN established strategies for maximization of space-time yield as well as total product titer for methanol-free protein production using UNLOCK PICHIA®.
The most widely used approach for recombinant protein production using Pichia pastoris as host is to express the gene of interest under control of AOX1 promoter applying methanol induction. However, many companies prefer not to use methanol due to safety concerns (e.g. for medical, food and feed products). To address this issue, VALIDOGEN has developed a set of AOX1 promoter variants that enable methanol-free protein production at multi-gram-per-liter titers. Methanol-free fermentation processes offer several advantages over those with methanol induction, such as low safety issues due to omittance of hazardous compounds, more economical production plant requirements and lower oxygen demand. Using components of VALIDOGEN’s UNLOCK PICHIA toolbox and process development methods, we established strategies for maximization of space-time yield as well as total product titer. Examples and case studies will be presented that show the applications of high-productivity methanol-free protein production processes with Pichia pastoris at various scales.
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